Enhance the Biodegradation of Anthraceneby Mutation from Bacillus species

Abstract

Anthracene, a tricyclic aromatic hydrocarbon causes many problems associated with health and environmental impact. It results from incomplete combustion of fuels present in automobiles and found in high concentrations in the polycyclic aromatic hydrocarbon (PAH)-contaminated sediments, surface soils & waste sites. Anthracene serves as a signature compound to detect PAH contamination, since its chemical structure is found in carcinogenic  PAHs. Therefore, the present study aimed to isolate bacteria capable of utilizing anthracene as the sole source of carbon and energy. Nine organisms was isolated among the 40 soil samples collected from (industrial area), Bangalore, based on the screening test the organism was selected and identified by 16s RNA sequencing. The organism subjected for optimise at different concentrations of anthracene, pH, temperatures, effect of carbon source and nitrogen source. After optimistaion, the more degradation was showing concentration at 10mg, at pH 5 and temperature at 35oC but effect of nitrogen source, sodium nitrate was showing 60times more degradation and along with that effect of carbon source was increasing nearly 15 times. After UV mutation, the degradation was increased nearly 20 times. The cytochrome oxidase activity was determined which is involved in the degradation of anthracene and the degradation was analysed by high performance liquid chromatography (HPLC).