Molecular Approach to Repair Damage on Streptozotocin Induced Diabetic Rats

Abstract

Streptozotocin- induced diabetic rats used as model to design drug for the treatment of diabetes and its related cardiac disease. The research was designed to investigate the differential expression of specific gene in diabetic rats treated with novel protein isolated from extract of Eugenia jambolana seeds used as trial therapeutic compound for drug development. Diabetes increases oxidant stress and doubles the risk of dying after myocardial infarction, but the mechanisms underlying increased mortality are unknown. Rats with streptozotocin-induced diabetes developed profound heart rate slowing and doubled mortality compared with controls after myocardial infarction Streptozotocin induces diabetic rat treated with Eugenia jambolana extracts had increased cell survival, maintained normal heart rates, and were resistant to diabetes-attributable mortality after myocardial infarction. Heart tissues from STZ induced diabetic rats were subjected to RNA extraction for gene expression
by using real time RT-PCR. Three diabetic cardiac-specific genes of interest such as IGF-1, VEGF and ANG-1 were chosen and the expression level of these genes has examined and the abnormal expression of genes in STZ induced diabetic group would be rescued by the protein of Eugenia jambolana based therapy. Gene expression of VEGF, ANG-1 and IGF-1 was upregulated after administrated protein of Eugenia jambolana (EJ) evidenced by RT-PCR. Administration of protein of Eugenia jambolana to diabetic rats significantly enhances survival, proliferation, and the angiogenic ability to improved function in a diabetic animals confirmed plant protein might be useful for the management of the diabetic cardiomyopathy